By - Jon Brown

Nitrification & Denitrification in Activated Sludge Treatment
May 4-5, 2021 | Online :: Central Time

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Ammonia removal is becoming more common in Wastewater plant discharge permits and is one of the most important and challenging process to maintain. Nitrification is the oxidation of that ammonia by converting it, in a two-step process, to nitrate using aerobic bacteria. Denitrification occurs in an anoxic environment where bacteria use the oxygen, from the nitrates, as they consume BOD and the nitrogen is released into the air. This process sounds simple but mastering it on a consistent basis can be difficult even for the most experienced operator.

The EUCI Nitrification & Denitrification in Activated Sludge Treatment course will review the general treatment operations and protocol for nitrification and denitrification wastewater treatment. This basic to intermediate course has been developed for all professionals in the wastewater field to provide an understanding of how this process can be applied to various wastewater plants. Proper operation and maintenance of the Nitrification process in biological and municipal wastewater treatment facilities can add to the plant’s sustainable use and reuse of water.

Learning Outcomes

  • Describe and identify the Activated Sludge process elements: components, requirements, differing designs, mathematical equations, etc.
  • Practice the manipulation of the Activated Sludge process to remove nutrients through nitrification and denitrification or phosphorus uptake
  • Review how inventory control (SRT) effects nutrient removal and disinfection
  • Detect and mitigate common challenges faced in daily wastewater plant operations
  • Calculate important equations relating to both BOD and nutrient removal
  • Utilize the chemistry basics of Nitrification (like what NH3 -> NO2->NO3 means)
  • Troubleshoot common problems relating to nutrient removal and activated sludge



EUCI has been accredited as an Authorized Provider by the International Association for Continuing Education and Training (IACET).  In obtaining this accreditation, EUCI has demonstrated that it  complies with the ANSI/IACET Standard which is recognized internationally as a standard of good practice. As a result of their Authorized Provider status, EUCI is authorized to offer IACET CEUs for its programs that qualify under the ANSI/IACET Standard.

EUCI is authorized by IACET to offer 1.0 CEUs for this event.


Requirements for Successful Completion of Program:

Participants must log in each day and be in attendance for the entirety of the course to be eligible for continuing education credit.

Instructional Methods

PowerPoint presentations, classroom discussions, and question-and-answer sessions will be used in this event.

Who Should Attend

POTW Operators, Industrial Wastewater Pretreatment Operators, Design Engineers, Prof. Eng/EIT, Industrial Plant (Manufacturing) Managers


Tuesday, May 4, 2021, Central Time

12:30 – 1:00 p.m. :: Login and Welcome

1:00 – 4:00 p.m. :: Course Timing

Activated Sludge, Part 1

  • What is the Goal of Nitrification and Denitrification in Activated Sludge?
  • Activated Sludge Basics
    • BOD
    • TSS
  • Definitions of Key Terms
  • Preliminary Treatment
    • Bar Racks
    • Screens
  • Primary Treatment
    • Clarifier
    • Solids Removal
  • Secondary Treatment
    • Aeration tank designs
    • SRT/MCRT/F to M
    • Nitrification
    • MLSS
    • SVI
    • O.
    • RAS rates
    • WAS rates
    • Clarifiers
    • Dewatering

Wednesday, May 5, 2021, Central Time

8:30 – 9:00 a.m. :: Login and Welcome

9:00 a.m. – 4:00 p.m. :: Course Timing

12:00 – 12:30 p.m. :: Lunch Break

Activated Sludge, Part 2

  • Disinfection
    • Chlorine
    • UV
    • Contact tanks
    • Dichlorination
  • Math: Basic Lab Calculations
  • Troubleshooting
    • Toxicity issues with maintenance of the biomass
    • Heavy metals removal upstream,
    • Proper influent control via pretreatment permitting
  • Documentation

Nitrification & Denitrification in Activated Sludge Treatment, Part 1

  • Overview of Nitrification and Denitrification
    • Why do we nitrify?
    • Forms of nitrogen in Wastewater
    • Environmental effects
  • Definitions of Key Terms
  • Types of Removal Systems
    • Physical
    • Chemical
    • Biological
  • Biological Nutrient Removal
    • Types of Nitrogen
    • How is this accomplished?
    • Ammonia to Nitrite
    • Nitrite to Nitrate
    • Denitrification

Lunch Break

Nitrification & Denitrification in Activated Sludge Treatment, Part 2

  • Equipment
    • Fixed film
    • Aeration
  • Conditions Needed
    • Temperature
    • SRT
    • D.O
    • S.R.P
  • Problems and Troubleshooting
    • Breakpoint chlorination
    • Rising sludge
    • Decreasing pH
  • Math: Basic Lab Calculations
  • Real World Examples
  • Phosphorus Removal
    • Chemical
    • Biological
    • How is this accomplished?


Ryan Staples, Senior Wastewater Operator at Portland Water District

Ryan Staples is a Senior Wastewater Operator with 9+ years of experience in wastewater management and public utilities. With a background as a Maintenance Wastewater Operator and a Control Center Operator for a large public water district, Ryan has experience efficiently operating four different activated sludge treatment plants. He has been involved in major plant upgrades, creating new and efficient SOP’s, asset management implementation, and monitoring, interpreting, modifying and reporting data.

Online Delivery

We will be using Microsoft Teams to facilitate your participation in the upcoming event. You do not need to have an existing Teams account in order to participate in the broadcast – the course will play in your browser and you will have the option of using a microphone to speak with the room and ask questions, or type any questions in via the chat window and our on-site representative will relay your question to the instructor.

  • IMPORTANT NOTE: After November 30 you will not be able to join a Teams meeting using Internet Explorer 11. Microsoft recommends downloading and installing the Teams app if possible. You may also use the Edge browser or Chrome.
  • You will receive a meeting invitation will include a link to join the meeting.
  • Separate meeting invitations will be sent for the morning and afternoon sessions of the course.
    • You will need to join the appropriate meeting at the appropriate time.
  • If you are using a microphone, please ensure that it is muted until such time as you need to ask a question.
  • The remote meeting connection will be open approximately 30 minutes before the start of the course. We encourage you to connect as early as possible in case you experience any unforeseen problems.


Please Note: This event is being conducted entirely online. All attendees will connect and attend from their computer, one connection per purchase. For details please see our FAQ

If you are unable to attend at the scheduled date and time, we make recordings available to all registrants for three business days after the event

Event Standard RateAttendees
Single Connection - Nitrification & Denitrification in Activated Sludge TreatmentUS $ 1195.00
Pack of 5 connectionsUS $ 4,780.00
Pack of 10 ConnectionsUS $ 8,365.00
Pack of 20 ConnectionsUS $ 14,340.00
Call us at 303.770.8800 if you have any specific questions on the volume discounts
* all other discounts do not apply to license packs

This event has the following related events:

Membrane Filtration for Drinking WaterUS $ 1195.00
Pack of 5 connectionsUS $ 4,780.00
Pack of 10 ConnectionsUS $ 8,365.00
Pack of 20 ConnectionsUS $ 14,340.00

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Take advantage of these discounts!

  • Attend the Course and Membrane Filtration for Drinking Water and pay US $ 2,195.00 per attendee (save US $ 195.00 each)

Cancellation Policy

Your registration may be transferred to a member of your organization up to 24 hours in advance of the event. Cancellations must be received on or before April 02, 2021 in order to be refunded and will be subject to a US $195.00 processing fee per registrant. No refunds will be made after this date. Cancellations received after this date will create a credit of the tuition (less processing fee) good toward any other EUCI event. This credit will be good for six months from the cancellation date. In the event of non-attendance, all registration fees will be forfeited. In case of conference cancellation, EUCIs liability is limited to refund of the event registration fee only. For more information regarding administrative policies, such as complaints and refunds, please contact our offices at 303-770-8800

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